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Image Search Results
Journal: PLoS ONE
Article Title: Peripheral Blood Invariant Natural Killer T Cells of Pig-Tailed Macaques
doi: 10.1371/journal.pone.0048166
Figure Lengend Snippet: (A) Flow cytometric figure shows the pattern of CD4+ versus CD8+ expression by α-GalCer-activated, IFN-γ-secreting i NKT cells gated from the PBMCs from one representative pig-tailed macaque. v(B) The graph shows the percentages of CD4+ or CD8+ i NKT cells among total IFN-γ secreting i NKT cells derived from the PBMCs of five pig-tailed macaques. v(C) The relative number of α-GalCer-activated, IFN-γ-secreting i NKT cells among PBMCs. In this assay, 5×10 5 pig-tailed macaque PBMC cells were stimulated with 0.1 µg/ml or 1 µg/ml of α-GalCer, and the relative numbers of IFN-γ-secreting cells were determined by an ELISpot assay. The results are expressed as the mean ± SD of triplicated wells.
Article Snippet: Finally, the spots were developed with an
Techniques: Expressing, Derivative Assay, Enzyme-linked Immunospot
Journal: PLoS ONE
Article Title: Peripheral Blood Invariant Natural Killer T Cells of Pig-Tailed Macaques
doi: 10.1371/journal.pone.0048166
Figure Lengend Snippet: (A) The relative numbers of IFN-γ secreting cells among PBMCs (ELISpot assay) and the percentage of α-GalCer-CD1d-Tet + CD3 + i NKT cells or CD8+ i NKT cells among PBMCs from each pig-tailed macaque (FACS analysis) are scatter-plotted to evaluate the correlation between these variables, and linear regression analysis was applied. (B) The relative numbers of IFN-γ secreting cells among PBMCs (ELISpot assay) and the percentage of CD4+ IFN-γ-secreting i NKT cells or CD8+ IFN-γ-secreting i NKT cells among PBMCs from each pig-tailed macaque (ICCS assay) are scatter-plotted to evaluate the correlation between these variables, and linear regression analysis was applied.
Article Snippet: Finally, the spots were developed with an
Techniques: Enzyme-linked Immunospot